Purpose: The purpose of this project is to find out the effect of the enzyme activity by looking at enzyme concentration, substrate concentration, and the effect of pH and temperature on enzyme activity.
Hypothesis: If procedures are followed correctly then the effect on enzyme activity will be found.
2. Set up 5 vials containing 40ml of 4 % hydrogen peroxide in each. Measure and record the depth of the hydrogen peroxide in the vials.
3. Dilute the enzyme as follows. Make each dilution in a separate cup.
100 units/ml = 12 ml 100 units/ml + 3 ml cold dH2O
4. What is the effect of temperature on enzyme activity?
As the time of the enzyme activity decreases the temperature increases,
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