Bio Lab
Every minute there are thousands of chemical reactions occurring in cells that are controlled by enzymes. (Vodopich) Enzymes are biological catalysts that speed up chemical reactions. As catalysts, enzymes lower the amount of energy needed to trigger a reaction. Enzymes are proteins with their own shapes determined by amino acid structures. The active site complexes on these structures determine what specific changes a substrate (reactant molecule in a catalyzed enzyme) will go through becoming a different substance with a different shape. (Weiss 2001) During this experiment the enzyme catalase was used to reduce hydrogen peroxide (H2O2) to water and oxygen. After adding aliqouts of reaction mixture we removed small amounts of sample at different time intervals we analyzed the molar concentration of H2O2 and the velocity of molar change per minute. Assaying denatured enzyme samples at different time intervals of 0, 0.5,1,1.5,and 2 minutes to review spectrophotometer readings of the corresponding samples of H2O2. Our absorbency readings at 500 decreased from .921 at 0 minutes to 0.086 at 2.0 minutes. The concentration of H2O2 also decreased according to time, from .28 moles at 0 minutes to 0.026 moles at 2 minutes. B
The molar concentration of hydrogen peroxide decreased according to tome as well, from 0.28 M H2O2 at 0 min to 0.026 M H2O2 at 2 minutes. Graph two , Molar concentration of H2O2 vs. time, also depicted a linear relationship. The longer the substrate was exposed to the reaction cocktail the rate of conservation of H2O2 decreased. The velocity of molarity change per minute peaked at 0.5 to 1 minute with a velocity of 0.1 and eventually rose to 0.16 at 1.5-2.0 minutes. Demonstrating that enzyme reaction increase with adequate substrates. 2. Velocity or rate of Molarity change/per minute was determined by the D. Read the instrument with at 100% T
Some common words found in the essay are:
Vodopich Enzymes, VI Discussion, Graph Molar, Reactions Add, Tubes Label, Introduction Enzymes, Potassium Iodide, H2O2 Dropping, Rate Molarity, McGraw-Hill Enzyme, 03 ml, molar concentration, rxn tube, 2 minutes, enzyme catalase, weiss 2001, remove 03 ml, concentration h2o2, remove 03, ml rxn tube, reaction cocktail, assay solution, molar concentration h2o2, 03 ml rxn, mix remove 03,
Approximate Word count = 1325
Approximate Pages = 5 (250 words per page double spaced)
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