Centrifugation Lab

A detailed Summary of Centrifugation Lab


The purpose of this experiment was to separate and identify the lower density particles in a homogenous mixture. Thus, the objective of the exercise was to realize that there is more than one structure in the cytoplasm and to isolate them using homogenization and differential centrifugation. In doing so, one can recognize and identify these particles by how the separate-which can be affected by density, shape, viscosity, the speed and/or diameter of the centrifuge. By examining the effects of the centrifugation, it allows you to see these principles for yourself, which gives you a better understanding of how the exercise works.

Ø Homogenization: break up pea cells in a blender to release organelles


S Test tube C (control tube)-sucrose buffer 2/3 full

After the first centrifugation, an examination of the bottom sediment layer was performed from the test tube set aside for observation. Using a long-tipped pipette, a small drop was placed on a glass slide and stained with iodine solution. Observed under low and high power through a microscope, the presence of starch was abundant. These grains are smaller than that of the starches present in the debris from the filtration. This is because they were too small to be filtered.

4. The results of this experiment illustrated the understanding of how structures separate by size, shape, and weight.

o Wait for one to turn pink (indicates presence of mitochondria)

S Test tube A-supernatant from centrifugation 2/3 full

During the first centrifugation (see diagram A) an examination of the residue left on the cheesecloth from the filtration process (see diagram B) was performed. A small amount with a drop of water was placed on a slide and examined under

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Approximate Word count = 684
Approximate Pages = 3 (250 words per page double spaced)

Category: Science

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