Bromelain is an organic catalyst that speeds up the rate of chemical reactions. Bromelain is used in this experiment to catalyze the break down of proteins and is called a proteolytic enzyme. Enzymes enable reactions to occur at a lower temperature and energy levels. This allows many reactions to occur inside living organisms that would otherwise have been impossible to take place. This enzyme is found in fruits, leaves, and stems of many species of pineapple. Pineapple is a tropical fruit native to northern South America. It is referred to as a multiple fruit because it consists of enlarged ovaries of many flowers more or less grown together into one mass. Ananas comosus is the type of pineapple used in this experiment. The bromelain is found in the pineapple attached itself to the substrate and broke it apart. The time required to complete the disassociation of the substrate was very important to the results of the lab, therefore time keeping must be precise in the experimentation. The effects of bromelain was compared under different temperatures and pH levels, the effects of bromelain were also observed in gelatin, there were three experiments performed and th

In the second experiment, effect of bromelain on gelation of gelatin, four clean test tubes were attained. The first test tube was labeled "fresh juice" and filled with one ml of fresh pineapple juice using a Pasteur pipette. The second test tube was labeled "hot juice" and then filled with one ml of the pineapple juice heated to 70 degrees C using a Pasteur pipette. The third test tube was labeled "canned juice" and then filled with one ml of canned pineapple juice using a Pasteur pipette. A fourth test tube was labeled "control" and filled with the pipette to one ml with water and dispensed into the test tube. Next, one ml of JELL-O was added to all four test tubes using a Pasteur pipette and the contents of the all four test tubes were mixed by placing a piece of wax paper over each tube and shaking it. Then all four test tubes were placed in a beaker of ice for five minutes. After five minutes in the ice, all four test tubes were removed and tilted slightly. The consistency of the gelatin in each test tube was observed and recorded in Table 2. The unused ice was then returned into the cooler. The test tubes were then emptied and washed out. The results of this experiment were that three test tubes turned to gel and one was still a liquid. The only one that didn't gel was the test tube which contained fresh pineapple juice.In the third experiment, effect of pH on rate of enzyme activity, ten clean test tubes were attained and labeled one through ten. Then they were filled with the following:Tube 1: 5ml of pineapple juice and 2ml of 1M HCltube 2: 5ml of water and 2ml of 1M HCL Tube 3: 5ml of pineapple juice Tube 4: 5ml of water and 2ml of pH 3.5 buffer Tube 5: 5ml of pineapple juice and 2ml of pH 5 buffer Tube 6: 5ml of water and 2ml of pH 5 buffer Tube 7: 5ml of pineapple juice and 2ml of pH 7 buffer Tube 8: 5ml of water and 2ml of pH 7 buffer Tume 9: 5ml of pineapple juice and 2ml of 1M NaOH Tube 10: 5ml of water and 2ml of 1M NaOH Each test tube was quickly inverted with a piece of wax paper covering the oppening to mix the solution. The solutions were then tested for pH levels using pH paper. These levels were recorded on the data chart. The ten test tubes were placed in 45 degrees celsius water bath. It important that the temperature remain constant. After five minutes, allowing the temperature inside the test tube to reach 45 degrees, a film strip was placed into each test tube. Every minute the strip were shaken vigorously for five seconds and cheched for clearing. This was done unyill the strips had reacted. Tubes 1,3,5,7, and 9 were the experimental and tubes 2,4,6,8, and 10 were the corresponding control tubes. As soon as the experimental tubes reacted the control group were considered done. The observations were recorded on the table 3. results In the first experiment the tubes containing juice reacted in 20 minutes at 20 degrees celsius, in 11 minutes at 30 degrees celsius, in 5 minutes at 40 degrees celsius, in 3 minutes at 50 degrees c

Some common words found in the essay are:
Gelatin Experiment, Reaction Rate, NaOH Tube, South America, HCL Tube, BromelainIntroduction Bromelain, test tubes, test tube, pineapple juice, 50 degrees, 70 degrees, fresh pineapple, five minutes, degrees celsius, juice gelatin, 5ml pineapple juice, 20 degrees, 5ml water 2ml, using pasteur pipette, test tube labeled, pineapple juice 2ml, Effects Temperature,
Approximate Word count = 2019
Approximate Pages = 8 (250 words per page double spaced)