Polymerase chain Reaction

A detailed Summary of Polymerase chain Reaction


In this experiment polymerase chain reaction (PCR) is used to amplify a

short piece of DNA from the human chromosome 8. A short insertion of a DNA

sequence, in this case Alu, which is found to be within the "tissue plasminogen

activator gene on chromosome 8, is observed very carefully. Even though the DNA

from human to human does not differ a whole lot and has many similarities the

diversity among regions of the human chromosomes is high. Because of the fact

there is a variety a sequences of TPA-25, it is said to be "polymorphic" meaning many forms are possible. This polymorphism helps us define the basis for diagnosis of genetic deseases, forensic identification, and paternity testing.

An Alu is a short interspersed, repetition of DNA elements that are scattered throughout the


In this experiment PCR will do the job to detect whether a person is

The concept behind Electrophoresis is two identify the genotype of a

positive for the TPA-25 insertion. PCR is a process in which a particular DNA

readily analyzed sample of a piece of DNA. The DNA is then denatured to break the bonds between the strands of the DNA. When it cools, the primers bind to the separated strands, and DNA polymerase quickly builds a new strand by joining the free nucleotide bases to the primers. When this process is repeated, a strand that was formed with one primer binds to the other primer, resulting in a new strand that is restricted solely to the desired segment. Thus, the region of DNA between the primers is selectively replicated. This lab process was developed in 1985 by Kary B. Mullis and used mostly in fingerprin

Some common words found in the essay are:
Introduction PCR, TPA25 Agarose, DNA DNA, Kary Mullis, PCR Electrophoresis, alu element, heterozygous meaning allele, meaning allele doesn't, plasminogen activator gene, tissue plasminogen activator, plasminogen activator, agarose gel, allele doesn't, meaning allele, chromosome 8, heterozygous meaning, strands dna, activator gene, tissue plasminogen,

Approximate Word count = 557
Approximate Pages = 2 (250 words per page double spaced)

Category: Science

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