Aim: An investigation into the factors affecting the activity of the enzyme Amylase - Planning

I predict that as the temperature increases, the speed of the reaction will increase. When a particular temperature is reached, I believe the rate of reaction will dramatically decrease. I believe this because most chemical reaction happens faster when the temperature is higher. At higher temperatures molecules mover around faster, which makes it easier for them to react together? Usually, a rise of 10 degrees celius will double the rate of reaction. This is true for enzymes up to about 40 degrees celius. However at 40 degrees celius the enzyme begins to be damaged, so the reaction slows. By 60 degrees celius, the enzyme is completely denatured. I predict that the same will happen the further away the pH is from pH 7. I believe this because the optimum temperature for most enzymes is about pH 7 therefore the further away from pH 7 (either more alkaline or more acidic) the less affective the enzyme.

To investigate the factors affecting the activity of the enzyme Amylase, I will adapt a pilot experiment, which investigates if the enzyme amylase break

In my design, I predicted that as the temperature increases, the speed of the reaction would increase. When a specific temperature was reached, I believed that the rate of reaction would dramatically decrease. I believed this because most chemical reactions happen faster when the temperature is higher. At higher temperatures molecules move around faster, which makes it easier for them to react together. Usually, a rise of 10 degrees celius will double the rate of reaction. This is true for enzymes up to about 40 degrees celius. However at 40 degrees celius the enzyme begins to be damaged, so the reaction slows down. By 60 degrees celius the enzyme is completely denatured. I predicted that the same would happen the further away the pH is from pH 7. I believed this because the optimum temperature for most enzymes is about pH 7 therefore the further away from pH 7 (either more alkaline or more acidic) the less effective the enzyme.

My results support most of the prediction since as the pH/temperature increases the rate of reaction increases until a point is reached and then the rate of reaction decreases. However, the optimum temperature was not 40 OC but 55 OC (unusually high) and the optimum pH was pH 5 not pH7. I also predicted that a rise of 10 OC would double the rate of reaction; this was not correct. As is evident in the table above, the time taken does not halve for every 10 degrees celius increase in temperature. I could not find a linear relationship between the temperature and rate of reaction, however with enough results (from a very large range of temperatures) a parabola could be made on Graph 1, it would then be possible to find a mathematical formula for the construction of the parabola and hence find the relationship between the temperature and rate of reaction. At current I do not have enough reliable evidence to draw any conclusions more specific than the rate of reaction is proportional to the temperature until 55 degrees celius is reached and after this point the temperature is inversely proportional to the rate of reaction.

2. Half fill another test tube with a 4% starch solution.

Some common words found in the essay are:
Adapted Experiment, Enzymes Substances, Evaluating Evidence, Note Optimum, Pilot Experiment, Planning Prediction, degrees celius, test tube, starch solution, rate reaction, enzyme amylase, solution test tube, solution test, ph 7, Amylase Aim, 40 degrees celius, active site, 40 degrees, 10 degrees, 10 degrees celius, amylase digest starch, pour amylase solution, ,
Approximate Word count = 2408
Approximate Pages = 10 (250 words per page double spaced)