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Enzyme Action Testing Catalase Activity

Enzyme Action: Testing Catalase Activity

Enzymes are proteins that serve as catalysts and accelerate chemical reactions in living organisms. Enzymes accomplish this by lowering the energy of activation of the organism it is acting upon, however enzymes will only lower the energy of activation for specific organisms, reducing chaotic chemical reactions. The reaction is carried out in it's entirety in the active site of the enzyme. The substrate (organism acted upon), binds itself into the active site, of the enzyme, and the chemical process begins. The enzyme's active site will reform itself for the substrate in order to form a perfect bond, this is called induced fit. This is only one of the changes that could occur to accommodate the substrate. For example, the enzyme might change the pH levels to promote the reaction.

There are numerous factors that can affect the rate of the chemical changes, four have the greatest affect. They are the concentration of the enzyme, the temperature, the pH level, and the salt concentration. The higher the concentration of the enzyme the faster the rate of change there is upon the organism. The temperature affects the rate in this way; each enzyme has a specific


The purpose of this experiment was to investigate the effects that enzyme concentration, temperature, and pH had on the rate at which enzyme catalyzed reactions took place. The results of this experiment can be summarized as follows: This enzyme like others increases the rate of chemical change with an increase of concentration. This specific enzyme has an optimum temperature around 30-35 Celsius. Finally, the enzyme has an optimum pH level of 10. However we cannot assume that the higher pH level there is the higher the reaction rate. More tests with different pH levels would have to be performed. This is the same case for the concentration of the enzyme, in order to say the enzyme functions better at a higher concentration more experiments with a higher concentration rate would have to be performed. For the time being, however it is safe to assume a steady increase in the rate of reaction with respect to an increase in concentration. A small amount of human error was included within this experiment, for example shaking (inadvertently) the test tube while gas pressure was being measured. However these errors weren't significant enough to have influential effects on the overall results of these experiments.

Data that was collected for this experiment on the rate of chemical reactions is summarized in the following tables, and the graphs attached.

There are very few factors that influence the function of the enzyme. Some enzymes require cofactors, substances that assist the chemical reaction. Chemical inhibitors can inhibit the enzyme's functionality (1) by attaching itself to the active site (competitive inhibitors) blocking the substrate; (2) by attaching itself to the enzyme in a place other than the active site, and changing the shape of the active site, thus disabling the active site. In this experiment, some of theses factors will be tested, concentration, hea

Some common words found in the essay are:
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Approximate Word count = 1277
Approximate Pages = 5 (250 words per page double spaced)


  

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