Effect of concentration of H2O2 on the enzyme catalase
Enzymes such as Catalase are large protein molecules that are found in living cells. They are used to speed up specific reactions in the cells. They are all specific as each enzyme just performs one particular reaction. In their globular structure, one or more polypeptide chains twist and fold, bringing together a small number of amino acids to form the active site, or the location on the enzyme where the substrate binds and the reaction takes place. Enzyme and substrate fail to join together if their shapes do not match exactly. This ensures that the enzyme does not participate in the wrong reaction. The enzyme itself is unaffected by the reaction. When the products have been released, the enzyme is ready to bind with a new substrate. Enzymes work to change the rate of a reaction without being absorbed by the reaction. The reactant that an enzyme acts on is called the enzyme's substrate. The product is what the enzymes convert the substrate into.
Catalase is an enzyme found in food such as potato and liver. It is used for removing Hydrogen Peroxide from the cells. Hydrogen Peroxide is the poisonous by-product of metabolism. Catalase speeds up the decomposition o
Pour the hydrogen peroxide into the conical flask and immediately insert the bung, at the same time start the stopwatch.
It would have been expected that when the concentration doubled so would the rate of reaction but this didn't occur. After 270 seconds the rate of reaction slows almost to a halt. The straight lines show this on the graphs. At this point virtually all of the active sites are occupied so the active sites are said to be saturated with hydrogen peroxide. Increasing the concentration at this point will not cause the rate of reaction to go up any more. . All the active sites are being used so any extra Hydrogen Peroxide molecules will have to wait until an active site becomes available.
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