Dna gel electrophorosis
DNA, Deoxyribonucleic acid, is a double stranded, helical nucleic acid molecule which determines inherited structure of a protein. The "steps" are made of bases: adenine, guanine, cytosine, and thymine. The sides are sugar and phosphate molecules. Restriction enzymes are enzymes that cut DNA at restriction sites, leaving fragments blunt or sticky. The restriction fragments are separated using a technique called DNA has a negative charge so when an electrical charge is applied it makes DNA move to the positive side. DNA is placed in agarose gel. Smaller fragments move faster. The purpose of this lab is to separate DNA fragments using gel electrophoresis. Hind III cuts AAGCTT between the two irst A's. EcoRI cuts at GAATTC between the G and the A. Hind III and EcoRI both make sticky ends. Our results for this lab were EcoRI separated into five fragments. Hind III separated into four fragments. The control only had one fragment.
the wells and expelled the contents. The top of the electrophoresis was used to load the lambda EcoRI, lambda Hind III, and lambda only
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Approximate Pages = 4 (250 words per page double spaced)
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