Dna gel electrophorosis

A detailed Summary of Dna gel electrophorosis

DNA, Deoxyribonucleic acid, is a double stranded, helical nucleic

acid molecule which determines inherited structure of a protein. The

"steps" are made of bases: adenine, guanine, cytosine, and thymine. The

sides are sugar and phosphate molecules. Restriction enzymes are

enzymes that cut DNA at restriction sites, leaving fragments blunt or

sticky. The restriction fragments are separated using a technique called

DNA has a negative charge so when an electrical charge is

applied it makes DNA move to the positive side. DNA is placed in

agarose gel. Smaller fragments move faster. The purpose of this lab is to

separate DNA fragments using gel electrophoresis. Hind III cuts AAGCTT

between the two irst A's. EcoRI cuts at GAATTC between the G and the

A. Hind III and EcoRI both make sticky ends.

Our results for this lab were EcoRI separated into five fragments.

Hind III separated into four fragments. The control only had one fragment.

the wells and expelled the contents. The top of the electrophoresis

was used to load the lambda EcoRI, lambda Hind III, and lambda only

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Category: Science

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