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BY01 Enzyme Catalase

The enzyme catalase speeds up the decomposition of Hydrogen Peroxide into water and oxygen as shown here, 2H2O2-------------------*2H2O+O2.

It is one of the fastest known enzymes and its turnover number is 6 million, which means the number of substrate molecules which one molecule of the enzyme turns to products per minute. This can be demonstrated by putting a piece of liver into a beaker of Hydrogen Peroxide, the fizzing shows a demonstration of the enzyme in action.

My aim is to examine how the concentration of the substrate hydrogen peroxide affects the enzyme catalase.

I am going to investigate the effect of varying the substrate concentration on enzyme catalase. I am going to use 8 different concentrations and record the time taken to collect 20ml of gas in the gas syringe. I will repeat all the 8 concentrations twice so I can see if they match, spot out any anonymous results and also I can work out the average time it takes to produce 20ml of gas at the certain concentrations. I will vary the concentrations by increasing and decreasing the amounts of Hydrogen Peroxide and water.

First of all I will ensure I have enough enzyme solution for the whole experimen


3. Set up the datalogging equipment as shown below.

6. Rinse the pH probe in the pH7 buffer solution and repeat experiment with a different milk sample.

The hydrolysis of milk fats by lipase



Some common words found in the essay are:
Hydrogen Peroxide, EVALUATION Looking, APPARATUS LIST, SAFETY ASPECTS, hydrogen peroxide, gas syringe, filter paper, produce 20ml, FAIR TEST, pieces liver, enzyme solution, 8 concentrations, glass wool, bile salts, mortar pestle, , produce 20ml oxygen, filter paper catalase, wool filter paper, paper catalase adsorbed, catalase adsorbed filter,
Approximate Word count = 2942
Approximate Pages = 12 (250 words per page double spaced)


  

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