ribosomes
Transport processes are based on the movement of matter when a force is exerted upon them (1). Diffusion, centrifugation, and electrophoresis are all based on forces causing movement or flow of particles. Diffusion is the migration of particles based on a concentration gradient. Sedimentation and electrophoresis are two transport processed in which the force exerted serves to separate particles. Sedimentation depends upon an increase in centrifugal force to cause a flow of matter. Electrophoresis is performed with an electrical field to provide the force necessary to cause the migration of particles through a medium. The purpose of this study is to use the transport processes of sedimentation and electrophoresis to isolate and examine certain components of prokaryotic and eukaryotic ribosomes. Using differential centrifugation, 70S E.coli Mre 600 ribosomes and 80S wheat germ ribosomes will be isolated. A protein extract from these ribosomes will be obtained by acetic acid/acetone precipitation and analyzed by a SDS-PAGE (Sodium dodecylsulfate polyacrylamide gel). A CHCl3 extraction method will be employed to isolate the RNA components of the ribosomes, which will be analyzed on an agarose gel. Standar
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Some common words found in the essay are:
Da Da, Data Wittmann, SDS-PAGE Specifically, SDS-PAGE Sodium, Introduction Transport, Treatment SDS, Lab Manual, Results SDS-PAGE, Rev Biochem, Ecoli Mre, molecular weight, nucleic acids, protein extract, agarose gel, rrna standard, weight range, bands observed, 5s 16s, molecular weight range, 16s 23s, 23s/16s rrna standard, figure 2, 5s 16s 23s, 5s rrna standard, ranging da da,
Approximate Word count = 2062
Approximate Pages = 8 (250 words per page double spaced)
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