Thimann and Koepfli identified growth-promoting and root-forming substances in plants. They demonstrated the practical use of IAA in the stimulation of root formation on cuttings. Even though there a number of studies indicating that auxins have a promotive effect on rooting there are exceptions which show that it either has no effect on root elongation or is inhibitory at higher concentrations. In addition to either exogenous or endogenous auxin effects it has been suggested that the sensitivity (responsiveness) of the cuttings to auxin may also be very important.
The proper environmental conditions and physiological status of the stock plant is very important for successful rooting of cuttings. Stock pla
The presence of leaves on the cutting has been shown to be beneficial to adventitious root formation, however, if the proper water relations are not maintained the cutting will not survive. Therefore, its important to maintain and atmosphere with a low evaporative demand on the plant to reduce transpirational loss, maintain temperatures which promotes root initiation while avoiding heat stress in the leaves and maintain light levels, suitable for photosynthesis and carbohydrate production once root initiation has occurred.
It is important to take into account that when IAA solutions are kept under nonsterile conditions and/or sunlight they will rapidly be destroyed, this is not a problem with synthetic auxins. The acid form of auxin is only soluble in
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