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DNA is the blueprint for all living things. DNA is made of a material called chromatin. The purpose of this experiment was to attempt to isolate, or separate, chromatin from onion cells. My lab partner was Dave Sugg.
Hypothesis: We should be able to isolate DNA from onion cells.
Procedure: To begin, weigh out about 15 grams of onion cubes and place it in a 400 ml beaker. Add about 60 ml of homogenizing medium to the onion cubes. Place it in a warm water bath for 8 minutes. Don't let it tip over!!! This softens onion tissue and allows homogenizing medium to penetrate the onion. The enzymes also get denatured. Quickly cool the solution to 15-20 degrees Celsius in an ice bath. Don't let it tip over!! Put the cooled solu
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onion, chromatin, degrees celsius, DNA extraction, ice cold, the dna, ethanol, experiment, Erlenmeyer flask, solution, the onion, high speed, cubes, minutes, proteins, whitish, filtrate, cheesecloth, blueprint, funnel, blender, cloudy, enzymes, Procedure, accessible, step, the experiment, glass, individual, more time, a film, general, the bottom, the filter, the blueprint,