Tyrosine Kinases

A detailed Summary of Tyrosine Kinases


In this experiment we will determine whether or not epidermal growth factors (EGF) stimulate target cells, through the receptor tyrosine kinase (RTK) (3). RTK's are transmembrane proteins that receive an extra cellular message, and transform it into an intracellular message. When inactive the RTK's reside as monomers in the lipid bylayer of the cell, until they are dimerized (2). At the same time of this dimerization the RTK then binds its ligand, EGF is a possible ligand. This action causes a conformational change that allows the RTK to be autophophorilated (1). Phosphorylation can happen on a few different side chains of the RTK. Such as serine, threonine, and tyrosine. We are concerned with the phosphorylation that occurs on the tyrosine. Phosphorylation of these side chains occurs at the exposed hydroxyl end of the amino acid. A hydrolysis reaction occurs and each dimmer yields phosphate groups to each other to allow the autophophorylation event (4).

By knowing that the tyrosine side chains are phosphorylated we can perform a western blot and then using a specific antibody we determine if our EGF has caused the RTK to be phosphorylated (3).


cells were originally serum starved to cause a low level of phosphorylation prior to the experiment; the cells could have been damaged. A number of things could have happened to the RTK's to either not allow them to bind ligand or not autophosphorylate.



Some common words found in the essay are:
SDS PAGE, , Methods Materials, western blot, blocking solution, placed top, 1 min washed, agitate 1 min, placed water, media cells, remove cells, transfer buffer, water bath, 150µl sds, blot using,

Approximate Word count = 843
Approximate Pages = 3 (250 words per page double spaced)

Category: Science

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