By knowing that the tyrosine side chains are phosphorylated we can perform a western blot and then using a specific antibody we determine if our EGF has caused the RTK to be phosphorylated (3).

cells were originally serum starved to cause a low level of phosphorylation prior to the experiment; the cells could have been damaged. A number of things could have happened to the RTK's to either not allow them to bind ligand or not autophosphorylate.

Some common words found in the essay are:
SDS PAGE, , Methods Materials, western blot, blocking solution, placed top, 1 min washed, agitate 1 min, placed water, media cells, remove cells, transfer buffer, water bath, 150µl sds, blot using,
Approximate Word count = 843
Approximate Pages = 3 (250 words per page double spaced)