Gel Electrophoresis

A detailed Summary of Gel Electrophoresis


In this investigation gel-electrophoresis is used in determining a killer's identity. DNA found under the victim's fingernails is compared to two possible suspects. The results ruled out Suspect 2 and victim himself. The bands from Suspect 1 matched the evidence taken from the fingernails.

Sarah Jeffries and Jim Candell had gone to a movie late in the evening and he needed to get some money from the ATM. Sarah stayed in the car while Jim got out to make the withdrawal. A man snuck up behind him and beat him to death with a tire iron. Sarah could not make his face out, but she did see his nose when he took his mask off. She said he had a Roman nose and possible scratch marks because Jim struggled with him. The police narrowed the suspects down to two and brought them in for DNA testing. This is a process of DNA testing is called gel-electrophoresis. DNA samples were taken from under Jim's fingernails. Then DNA was ta


The victim's DNA did not match the suspects' DNA; therefore, the DNA taken from the suspects would not have any confusion with the results. Suspect 2 did not match up with the evidence band, so that ruled out suspect 2, but does not make suspect 1 the killer. Suspect 1's bands did match up with the evidence though, proving that he was the killer. Suspect 1's bands matched the spacious bands of the evidence, unlike suspect 2.

First the gel was made from agarose. The agarose was placed in a microwave until melted. A comb is then placed in the gel at a vertical position. The gel box is then placed on a level surface, to be left undisturbed for about 20-30 minutes. 10 ml of molten agarose is poured evenly into the gel box to fill the central cavity and wrap around the wells. Then the gel was left alone for the time listed above. Agarose has a neutral charge. Using a neutral substance will not interfere with the electrical current moving through t

Some common words found in the essay are:
Materials Methods, ATM Sarah, Victim's DNA, DNA DNA, , Depending DNA, Jim Candell, suspect 2, gel box, ruled suspect 2, killer suspect 1's, poured gel box, suspect 1's bands, poured gel, ruled suspect, box placed, victim's dna, suspect 1, 10 ml, match evidence, dna testing,

Approximate Word count = 647
Approximate Pages = 3 (250 words per page double spaced)

Category: Science

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