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How to Find Starch, Protien and glucouse in Unkown Substance

Day 1: What organic or inorganic compounds (starch, protein, glucose, NACL) are in an unknown solution.

Day 2: Which of the substances (starch or NACL) will be able to diffuse out of the plastic bag or "cell" if you will.

Hypothesis: If the starch and NACL compounds do diffuse through the plastic bag, then we know that these substances will diffuse in an actual cell also.

Dialysis tubing, thread, 100 mL beaker, 10 mL graduated cylinder, unknown mixture, food test reagents, test tubes, hot water bath.

Step 1- Put on safety goggles and apron.

Step 2- First, set up four test tubes on the lab surface; held in place upright. Then pour 2 mL of the unknown solution into the first test tube. Then use the bottle of Biruet reagent to drop exactly five drops directly into the first test tube. Observe the color of the mixed solution. If it turned Indigo then we can conclude that the unknown solution does in fact contain protein.

Step 3- Second, pour exactly 2 mL of the unknown solution into the second test tube and then use the bottle of Iodine to drop exactly 3 mL directly into test tube two. If, after carefully observing the change in color; it turned a dark


Step 5- Turn the tubing upside down to make sure that there are no leaks.

Step 4- Then pour exactly 5 mL of the unknown solution directly into the tubing, leaving enough room so that you can tie the top tightly with a thread.

Step 1- First take a 12 cm piece of dialysis tubing which has been soaked in water.



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Approximate Word count = 1083
Approximate Pages = 4 (250 words per page double spaced)


  

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